84-5614-90　Superoxide Dismutase Cu,Zn (SOD) 100ug　544525

特徴

• Superoxide dismutase [Cu-Zn] (EC 1.15.1.1; UniProt P00441; also known as Cu/Zn superoxide dismutase, Epididymis secretory protein Li 44, hSod1, Indophenoloxidase A, Superoxide dismutase 1) is encoded by the SOD1 (also known as ALS1) gene (Gene ID 6647) in human.
• Superoxide dismutases (SOD) provide important cellular antioxidant defense against oxidative damage by catalyzing the dismutation (or partitioning) of the superoxide (O2−) radical into either molecular oxygen (O2) or hydrogen peroxide (H2O2).
• Superoxide dismutases are divided into three families based on the metal cofactor.
• The Cu/Zn type binds both copper and zinc, the Fe and Mn types bind either iron or manganese, and the Ni type binds nickel.
• Three forms of SOD are present in mammals, the cytosolic SOD1, the mitochondrial SOD2, and the extracellular SOD3.
• SOD1 exists in dimeric form, whereas the SOD2 and SOD3 are tetrameric.
• SOD1 and SOD3 Cu/Zn type, whereas SOD2 is Mn type.
• Mutations in SOD1 gene have been linked to 20% of amyotrophic lateral sclerosis (ALS), also known as Lou Gehrig disease.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1ug/ml detected Cu/Zn-SOD in 10ug of human brain tissue lysate
• Immunohistochemistry: 1:1000 detected Cu/Zn-SOD in human cerebral cortex, mouse cerebellum, and mouse kidney tissue
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.