Allophycocyanin, Crosslinked, Goat Anti-Rabbit IgG (H+L) is prepared from crosslinked allophycocyanin (APC) and an affinity purified goat anti-rabbit IgG antibody.
Since this phycobiliprotein is optimally excited at 633 nm, it can also be excited at 594 nm; therefore, it can be used with samples simultaneously labeled with Texas Red dye or Alexa Fluor 594 dye without the need of a second excitation source.
APC can be used in flow cytometry and imaging applications and is more photostable than Cy5 conjugates.
Applications:Suitable for use in Flow Cytometry, Immunocytochemistry and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 1-10ug/mlImmunofluorescence: 4ug/mlImmunocytochemistry: 4ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
仕様
Size：250ul
Host：goat
Source Antibody：rabbit
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.5, 5mM sodium azide. Labeled with Allophycocyanin (APC).
Specificity：Recognizes IgG (H+L) cross-adsorbed against human IgG and serum, mouse IgG and serum, and bovine serum.
Isotype：IgG
Calc Applications Abbrev：FC IC IF
Calc Crossreactivity：Rb
Immunogen：Gamma Immunoglobins Heavy and Light chains
