84-5614-43　［取扱停止］SARS-COV/SARS-COV-2 Spike RBD (2019-nCoV Coronavirus) 50ul　544126

特徴

• Protein S (PROS1) is glycoprotein and expressed in many cell types supporting its reported involvement in multiple biological processes that include coagulation, apoptosis, cancer development and progression, and the innate immune response.
• Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2, DPP4, CEACAM etc..
• The spike (S) glycoprotein of Coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process.
• Most notable is severe acute respiratory syndrome (SARS).
• The severe acute respiratory syndrome-Coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion.
• It is also a major immunogen and a target for entry inhibitors.
• It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor.
• The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2.
• S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor.
• S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:5000ELISA: 1:5000-1:10,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.