The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo.
It is composed of three glycoproteins with various functions during fertilization and preimplantation development.
The glycosylated mature peptide is one of the structural components of the zona pellucida and functions in secondary binding and penetration of acrosome-reacted spermatozoa.
Female mice lacking ZP2 (zona pellucida glycoprotein 2) do not form a stable zona matrix and are sterile.
Alternative splicing results in multiple transcript variants.
Applications:Suitable for use in Immunohistochemistry, ELISA.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:30ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity:Recognizes endogenous levels of human ZP2.
Isotype:IgG
Calc Applications Abbrev:E IHC
Calc Crossreactivity:Hu
Immunogen:Synthetic peptide corresponding to the Internal region of human ZP2.