YBX1 encodes a highly conserved cold shock domain protein that has broad nucleic acid binding properties.
The encoded protein functions as both a DNA and RNA binding protein and has been implicated in numerous cellular processes including regulation of transcription and translation, pre-mRNA splicing, DNA reparation and mRNA packaging.
This protein is also a component of messenger ribonucleoprotein (mRNP) complexes and may have a role in microRNA processing.
This protein can be secreted through non-classical pathways and functions as an extracellular mitogen.
Aberrant expression of the gene is associated with cancer proliferation in numerous tissues.
YBX1 may be a prognostic marker for poor outcome and drug resistance in certain cancers.
Alternate splicing results in multiple transcript variants.
Pseudogenes of YBX1 are found on multiple chromosomes.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human YB-1. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human YB-1 around the non-phosphorylation site of S102.
