84-5610-49　［取扱停止］WISP1 100ul　543803

特徴

• WISP1 encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family.
• WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes.
• The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain.
• This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation.
• It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors.
• The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation.
• It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase.
• It is 83% identical to the mouse protein at the amino acid level.
• Multiple alternatively spliced transcript variants have been identified.
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:5000-1:20,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.