VRK3 (vaccinia related kinase 3) encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases.
In both human and mouse, VRK3 has substitutions at several residues within the ATP binding motifs that in other kinases have been shown to be required for catalysis.
In vitro assays indicate the protein lacks phosphorylation activity.
The protein, however, likely retains its substrate binding capability.
VRK3 is widely expressed in human tissues and its protein localizes to the nucleus.
Alternative splicing results in multiple transcripts encoding different isoforms.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human VRK3.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to the Internal region of human VRK3.
