84-5608-64　［取扱停止］VE-Cadherin, phosphorylated (Tyr731) 30ul　543698

特徴

• Vascular endothelial (VE)-cadherin (VE-CAD), also called 7B4 and cadherin‑5 (CDH5), is a member of the cadherin family of cell adhesion molecules.
• Cadherins are calcium‑dependent transmembrane proteins which bind to one another in a homophilic manner.
• On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin).
• This association links the cadherin protein to the cytoskeleton.
• Without association with the catenins, the cadherins are non-adhesive.
• Cadherins play a role in development, specifically in tissue formation.
• They may also help to maintain tissue architecture in the adult.
• VE-cadherin has been shown to play important roles in vasculogenesis and angiogenesis.
• VE-cadherin is a classical cadherin molecule.
• Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short C-terminal cytoplasmic domain responsible for interacting with the catenins.
• Human VE-cadherin is a 784aa residue protein with a 25aa signal sequence and a 759aa propeptide.
• The mature protein begins at 48aa and has a 546aa extracellular domain, a 27aa transmembrane domain, and a 164aa cytoplasmic domain.
• The human and mouse mature VE-cadherin proteins share approximately 74% homology.
• Applications:Suitable for use in Western Blot, Immunohistochemistry, and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:20,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.