CDH5 encodes a classical cadherin of the cadherin superfamily.
The encoded preproprotein is proteolytically processed to generate the mature glycoprotein.
This calcium-dependent cell-cell adhesion molecule is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail.
Functioning as a classical cadherin by imparting to cells the ability to adhere in a homophilic manner, this protein plays a role in endothelial adherens junction assembly and maintenance.
CDH5 is located in a gene cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer.
Applications:Suitable for use in Western Blot, Immunofluorescence, Immunohistochemistry and ELISA.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:500-1:2000Immunofluorescence: 1:50-1:200Immunohistochemistry (paraffin): 1:50-1:300ELISA: 1:10,000-1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human VE-Cadherin at ~86kD. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to the Internal region of human VE-Cadherin
