The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates.
A wide range of enzymes facilitate the proteolytic Ub pathway, including USPs (ubiquitin specific peptidases), which are cysteine proteases that are responsible for the release of ubiquitin from a ubiquitylated substrate and precursor fusion proteins.
USP45 (Ubiquitin C-terminal hydrolase 45), also known as Deubiquitinating enzyme 45, is a 819aa protein that is involved in ubiquitin-dependent protein catabolism.
USP45 differs from other USPs in that it contains a UBP-type zinc finger, a domain which binds ubiquitin.
Although USP45 is broadly expressed, the highest levels can be found in skeletal muscle, spleen and ovary.
There are three isoforms of USP45 which are produced as a result of alternative splicing.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human USP45. Species Crossreactivity: Mouse and rat
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa270-350 from the Internal region of human USP45.
