UHRF1 encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases.
E3 ubiquitin-protein ligase UHRF1binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression.
Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle.
It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint.
It is regarded as a hub protein for the integration of epigenetic information.
This gene is up-regulated in various cancers, and it is therefore considered to be a therapeutic target.
Multiple transcript variants encoding different isoforms have been found for this gene.
A related pseudogene exists on chromosome 12.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human UHRF1.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to the Internal region of human UHRF1.
