Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP).
UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak.
UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane.
They also reduce the mitochondrial membrane potential in mammalian cells.
Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known.
UCPs contain the three homologous protein domains of MACPs.
UCP2 (uncoupling protein 2)is expressed in many tissues, with the greatest expression in skeletal muscle.
It is thought to play a role in nonshivering thermogenesis, obesity and diabetes.
Chromosomal order is 5'-UCP3-UCP2-3'.
Applications:Suitable for use in Immunohistochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human UCP2. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to the Internal region of human UCP2.
