Ubiquitin (Ub) is among the most phylogenetically conserved proteins known.
The primary function of ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S proteosome.
This small, 76aa protein can be covalently attached to cellular proteins via an isopeptide linkage between the C-terminal group of ubiquitin and lysine amino groups on the acceptor protein.
For proteolysis to occur, ubiquitin oligomers must be assembled.
Ubiquitin chains on proteolytic substrates are commonly found to have an isopeptide bridge between Lysine 48 of one ubiquitin molecule and the C-terminal of a neighboring ubiquitin molecule.
Ubiquitin also plays a role in regulating signal transduction cascades through the elimination inhibitory proteins, such as IkappaBalpha and p27.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human Ub. Species Crossreactivity: mouse, rat
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to the human Ub around the acetylation site of K48.
