84-5604-41　［取扱停止］TUT4 100ul　543467

特徴

• ZCCHC11 is an RNA uridyltransferase (EC 2.7.7.52) that uses UTP to add uridines to the 3-prime end of substrate RNA molecules.
• GO annotations related to this gene include nucleic acid binding and nucleotidyltransferase activity.
• Uridylyltransferase that mediates the terminal uridylation of mRNAs with short (less than 25 nucleotides) poly(A) tails, hence facilitating global mRNA decay.
• Involved in microRNA (miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets.
• Also acts as a suppressor of miRNA biogenesis by mediating the terminal uridylation of some miRNA precursors, including that of let-7 (pre-let-7), miR107, miR-143 and miR-200c.
• Uridylated miRNAs are not processed by Dicer and undergo degradation.
• Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cell pluripotency.
• Does not bind RNA directly, but recruited to RNA targets by RNA-binding protein LIN28A.
• Also catalyzes the 3 uridylation of miR-26A, a miRNA that targets IL6 transcript.
• This abrogates the silencing of IL6 transcript, hence promoting cytokine expression.
• May also suppress toll-like receptor-induced NF-kappa-B activation via binding to T2BP.
• Does not play a role in replication-dependent histone mRNA degradation.
• Due to functional redundancy between ZCCHC6 and ZCCHC11, the identification of the specific role of each of these proteins is difficult.
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:5000-1:20,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.