84-5604-01　Tubulin alpha 30ul　543446

特徴

• Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins.
• TUBA1A encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families.
• Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes.
• The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly.
• There are multiple alpha and beta tubulin genes, which are highly conserved among species.
• This gene encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes.
• Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.
• This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q.
• Mutations in this gene cause lissencephaly type 3 (LIS3) - a neurological condition characterized by microcephaly, mental retardation, and early-onset epilepsy and caused by defective neuronal migration.
• Alternative splicing results in multiple transcript variants encoding distinct isoforms.
• Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300Immunofluorescence: 1:200-1:1000ELISA: 1:5000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.