84-5594-45　［取扱停止］TBC1D4 30ul　542932

特徴

• TBC1D4 (TBC1 domain family member 4) is a member of the Tre-2/BUB2/CDC16 domain family.
• The protein encoded by TBC1D4 is a Rab-GTPase-activating protein, and contains two phopshotyrosine-binding domains (PTB1 and PTB2), a calmodulin-binding domain (CBD), a Rab-GTPase domain, and multiple AKT phosphomotifs.
• This protein is thought to play an important role in glucose homeostasis by regulating the insulin-dependent trafficking of the glucose transporter 4 (GLUT4), important for removing glucose from the bloodstream into skeletal muscle and fat tissues.
• Reduced expression of this gene results in an increase in GLUT4 levels at the plasma membrane, suggesting that this protein is important in intracellular retention of GLUT4 under basal conditions.
• When exposed to insulin, this protein is phosphorylated, dissociates from GLUT4 vesicles, resulting in increased GLUT4 at the cell surface, and enhanced glucose transport.
• Phosphorylation of this protein by AKT is required for proper translocation of GLUT4 to the cell surface.
• Individuals homozygous for a mutation in this gene are at higher risk for type 2 diabetes and have higher levels of circulating glucose and insulin levels after glucose ingestion.
• Alternative splicing results in multiple transcript variants encoding different isoforms.
• Applications:Suitable for use in Immunohistochemistry, Immunofluorescence and ELISA.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry (paraffin): 1:100-1:300Immunofluorescence: 1:200-1:1000ELISA: 1:10,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.