84-5592-89　［取扱停止］TAF II p18 100ul　542851

特徴

• Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides.
• The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals.
• TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs.
• TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation.
• TAF13 (TATA-box binding protein associated factor 13) encodes a small subunit associated with a subset of TFIID complexes.
• This subunit interacts with TBP and with two other small subunits of TFIID, TAF10 and TAF11.
• There is a pseudogene located on chromosome 6.
• Applications:Suitable for use in Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.