Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin.
Phosphorylation at Tyr694 is obligatory for Stat5 activation.
This phosphorylation is mediated by Src upon erythropoietin stimulation.
Stat5 is constitutively active in some leukemic cell types.
Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility.
Stat5a and Stat5b are independently regulated and activated in various cell types.
For instance, interferon treatment predominantly activates Stat5a in U-937 cells and Stat5b in HeLa cells.
Applications:Suitable for use in Western Blot, Immunohistochemistry, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human Stat5a. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human Stat5a around the non-phosphorylation site of S780.
