84-5589-27　［取扱停止］Stat5a 100ul　542667

特徴

• Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin.
• Phosphorylation at Tyr694 is obligatory for Stat5 activation.
• This phosphorylation is mediated by Src upon erythropoietin stimulation.
• Stat5 is constitutively active in some leukemic cell types.
• Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility.
• Stat5a and Stat5b are independently regulated and activated in various cell types.
• For instance, interferon treatment predominantly activates Stat5a in U-937 cells and Stat5b in HeLa cells.
• Applications:Suitable for use in Western Blot, Immunofluorescence, and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunofluorescence: 1:200-1:1000ELISA: 1:5000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.