The protein encoded by STAT4 (signal transducer and activator of transcription 4) is a member of the STAT family of transcription factors.
In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators.
This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells.
Mutations in STAT4 may be associated with systemic lupus erythematosus and rheumatoid arthritis.
Alternate splicing results in multiple transcript variants that encode the same protein.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation, ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300Immunoprecipitation: 2-5 ug/mg lysateELISA: 1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human Stat4.
Isotype：IgG
Calc Applications Abbrev：E IHC IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to human Stat4 around the non-phosphorylation site of Y693
