Signal transducer and activator of transcription 1 encoded by STAT1 is a member of the STAT protein family.
In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators.
Signal transducer and activator of transcription 1can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6.
Signal transducer and activator of transcription 1 mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens.
Two alternatively spliced transcript variants encoding distinct isoforms have been described.
Applications:Suitable for use in Western Blot, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunofluorescence: 1:50-1:200Immunohistochemistry (paraffin): 1:100-1:300Immunoprecipitation (lysate): 2-5ug/mgELISA: 1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human Stat1 protein only when phosphorylated at Y701. Species Crossreactivity: mouse, rat and monkey
Isotype：IgG
Calc Applications Abbrev：E IF IHC IP WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide derived from human Stat1 around the phosphorylation site of Y701.
