Signal transducing adaptor molecule 2 (STAM2) is a ubiquitously expressed STAM family adaptor protein and an integral component of the ESCRT-0 complex.
Similar to STAM1, STAM2 possesses a single SH3 domain and an immunoreceptor tyrosine-based activation motif (ITAM).
Following activation of multiple growth factor and cytokine cell surface receptors, the STAM2 protein undergoes tyrosine phosphorylation and potentiates mitogenic signals driven by these receptors.
Research studies demonstrate that STAM2 is localized to complexes containing Eps15, Hrs, and STAM1 proteins on early endosome membranes.
A tandem, N-terminal VHS (Vps27/Hrs/STAM) domain and UIM (ubiquitin-interacting) motif within STAM2 facilitate STAM2 interaction with ubiquitinated cargo proteins, suggesting that this adaptor participates in the endosomal sorting of ubiquitinated proteins targeted for lysosomal degradation.
Gene targeting studies have revealed an indispensible role for STAM2 in T-cell development.
Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human STAM2 only when phosphorylated at Y192. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to human STAM2 around the phosphorylation site of Y192.
