SP100 (SP100 nuclear antigen) encodes a subnuclear organelle and major component of the PML (promyelocytic leukemia)-SP100 nuclear bodies.
PML and SP100 are covalently modified by the SUMO-1 modifier, which is considered crucial to nuclear body interactions.
The encoded protein binds heterochromatin proteins and is thought to play a role in tumorigenesis, immunity, and gene regulation.
Alternatively spliced variants have been identified for SP100; one of which encodes a high-mobility group protein.
Applications:Suitable for use in Western Blot, Immunohistochemistry, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human SP-100.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa250-330 from the Internal region of human SP-100.
