84-5583-33　［取扱停止］Smad1, phosphorylated (Ser465) 30ul　542356

特徴

• SMAD family member 1 encoded by SMAD1 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C.
• elegans gene Sma.
• SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways.
• This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses.
• In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase.
• The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation.
• This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation.
• Alternatively spliced transcript variants encoding the same protein have been observed.
• Applications:Suitable for use in Western Blot, Immunohistochemistry, and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:20,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.