84-5583-13　［取扱停止］SLP-76, phosphorylated (Tyr128) 30ul　542346

特徴

• SLP-76 was originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation in the leukemic T cell line Jurkat.
• The SLP-76 locus has been localized to human chromosome 5q33 and the gene structure has been partially characterized in mice.
• The human and murine cDNAs both encode 533aa proteins that are 72% identical and comprised of three modular domains.
• The NH2-terminal contains an acidic region that includes a PEST domain and several tyrosine residues which are phosphorylated following TCR ligation.
• SLP-76 also contains a central proline-rich domain and a COOH-terminal SH2 domain.
• A number of additional proteins have been identified that associate with SLP-76 both constitutively and inducibly following receptor ligation, supporting the notion that SLP-76 functions as an adaptor or scaffold protein.
• Studies using SLP-76 deficient T cell lines or mice have provided strong evidence that SLP-76 plays a positive role in promoting T cell development and activation as well as mast cell and platelet function.
• Applications:Suitable for use in Western Blot, Immunohistochemistry (paraffin), ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:5000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.