84-5582-41　［取扱停止］Skp2 p45 100ul　542310

特徴

• SKP2 encodes a member of the F-box protein family which is characterized by an approximately 40aa motif, the F-box.
• The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination.
• The F-box proteins are divided into 3 classes: fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs.
• S-phase kinase-associated protein 2 encoded by this gene belongs to the Fbls class; in addition to an F-box, this protein contains 10 tandem leucine-rich repeats.
• This protein is an essential element of the cyclin A-CDK2 S-phase kinase.
• It specifically recognizes phosphorylated cyclin-dependent kinase inhibitor 1B (CDKN1B, also referred to as p27 or KIP1) predominantly in S phase and interacts with S-phase kinase-associated protein 1 (SKP1 or p19).
• In addition, this gene is established as a protooncogene causally involved in the pathogenesis of lymphomas.
• Alternative splicing of this gene generates three transcript variants encoding different isoforms.
• Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300Immunofluorescence: 1:200-1:1000ELISA: 1:20,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.