SIRPA encoded by Tyrosine-protein phosphatase non-receptor type substrate 1 is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily.
SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes.
This protein can be phosphorylated by tyrosine kinases.
The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs.
This protein was found to participate in signal transduction mediated by various growth factor receptors.
CD47 has been demonstrated to be a ligand for this receptor protein.
This gene and its product share very high similarity with several other members of the SIRP family.
These related genes are located in close proximity to each other on chromosome 20p13.
Multiple alternatively spliced transcript variants have been determined for this gene.
Applications:Suitable for use in Western Blot, Immunohistochemistry, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human SIRP-alpha1. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa420-500 of human SIRP-alpha1 at C-terminal.
