INPP5D is a member of the inositol polyphosphate-5-phosphatase (INPP5) family and encodes phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1 with an N-terminal SH2 domain, an inositol phosphatase domain, and two C-terminal protein interaction domains.
Expression of this protein is restricted to hematopoietic cells where its movement from the cytosol to the plasma membrane is mediated by tyrosine phosphorylation.
At the plasma membrane, the protein hydrolyzes the 5' phosphate from phosphatidylinositol (3,4,5)-trisphosphate and inositol-1,3,4,5-tetrakisphosphate, thereby affecting multiple signaling pathways.
The protein is also partly localized to the nucleus, where it may be involved in nuclear inositol phosphate signaling processes.
Overall, the protein functions as a negative regulator of myeloid cell proliferation and survival.
Mutations in this gene are associated with defects and cancers of the immune system.
Alternative splicing of this gene results in multiple transcript variants.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human SHIP-1. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa1110-1190 of human SHIP-1 at C-terminal.
