84-5581-43　［取扱停止］Shb, phosphorylated (Tyr246) 100ul　542261

特徴

• SHB (SH2 homology protein in B/ beta -cells) is a 55-59kD cytoplasmic adaptor protein that serves as a link between phosphotyrosine residues and downstream signaling pathways.
• SHB is ubiquitously expressed, and binds tyrosine kinase receptors such as FGFR1, VEGFR2, and the TCR (zeta -chain) following their activation.
• It is highly modular, and through a variety of motifs, is able to bind multiple, structurally unrelated proteins that collectively generate a signal transduction network.
• Human SHB is 509aa in length.
• It contains an N-terminal Pro-rich region that binds SH3 domain-containing proteins (aa19-45), a central PTB domain that binds select aa motifs, and a C-terminal SH2 domain that interacts with phosphotyrosines (aa410-504).
• There are at least nine utilized phosphorylation sites.
• There are two isoform variants.
• One is 66-68kD in size, and possesses an 87aa Pro-rich N-terminal extension.
• This MW may increase to 77-80kD following posttranslational processing.
• A second variant shows an 18 aa substitution for aa280-509.
• Over aa2-128, human SHB shares 91% aa identity with mouse SHB.
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:10,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.