The SH2 (Src homology 2) domain is a structurally conserved motif that contains two alpha helices and seven beta strands and is found in a variety of proteins that are involved in signal transduction throughout the cell.
Specifically, the SH2 domain targets SH2 domain-containing proteins to tyrosine-phosphorylated sites, an event that can trigger a protein-protein interaction cascade which may ultimately effect gene expression and cellular function.
Shb (SH2 domain-containing adapter protein b), Shd (SH2 domain-containing adapter protein d), She (SH2 domain-containing adapter protein e) and Shf (SH2 domain-containing adapter protein f) are SH2 domain-containing proteins that play various roles throughout the cell.
Shb is a widely expressed protein that localizes to both the cell membrane and the cytoplasm and plays an important role in signal transduction, mainly by linking activated proteins to downstream signaling targets, thereby propagating a signal cascade.
Unlike Shb, Shd and Shf are thought to function as adaptor proteins, the former of which may be involved in apoptotic regulation.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human Shb. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to aa380-460 from human Shb at C-terminal.
