SEPT5 (septin 5) is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins.
Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization.
Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells.
SEPT5 is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes.
A translocation involving the MLL gene and SEPT5 has also been reported in patients with acute myeloid leukemia.
Alternative splicing results in multiple transcript variants.
The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human Septin 5. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa180-260 of human Septin 5.
