Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters.
The protein encoded by CBF2alpha (runt related transcription factor 1, RUNX1) represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis.
Chromosomal translocations involving this gene are well-documented and have been associated with several types of leukemia.
Three transcript variants encoding different isoforms have been found for CBF2alpha.
Applications:Suitable for use in Western Blot, Immunohistochemistry, ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human RUNX1 (phospho Ser276). Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human RUNX1 around the phosphorylation site of S276.
