As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress.
It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism.
Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage (PubMed: 9430682).
In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation.
RPA3 (replication protein A3) is a protein coding gene.
Diseases associated with RPA3 include epidemic typhus and ovary serous adenocarcinoma.
Among its related pathways are mismatch repair and DNA double-strand break repair.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:10,000-1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human RPA14. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to aa30-80 from human RPA14.
