Nuclear receptor ROR-alpha encoded by RORA is a member of the NR1 subfamily of nuclear hormone receptors.
It can bind as a monomer or as a homodimer to hormone response elements upstream of several genes to enhance the expression of those genes.
The encoded protein has been shown to interact with NM23-2, a nucleoside diphosphate kinase involved in organogenesis and differentiation, as well as with NM23-1, the product of a tumor metastasis suppressor candidate gene.
Also, it has been shown to aid in the transcriptional regulation of some genes involved in circadian rhythm.
Four transcript variants encoding different isoforms have been described for this gene.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300Immunofluorescence: 1:200-1:1000ELISA: 1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human RORalpha. Species Crossreactivity: monkey
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mk
Immunogen：Synthetic peptide corresponding to aa20-100 of human RORalpha at N-terminal.
