Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit.
Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins.
RPL15 (ribosomal protein L15) encodes a ribosomal protein that is a component of the 60S subunit.
The protein belongs to the L15E family of ribosomal proteins.
It is located in the cytoplasm.
RPL15 shares sequence similarity with the yeast ribosomal protein YL10 gene.
Although this gene has been referred to as RPL10, its official symbol is RPL15.
RPL15 has been shown to be overexpressed in some esophageal tumors compared to normal matched tissues.
Alternate splicing results in multiple transcript variants.
As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of RPL15 dispersed through the genome.
Applications:Suitable for use in Western Blot, Immunohistochemistry, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human Ribosomal Protein L15. Species Crossreactivity: Mouse, Rat
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa10-90 from the Internal region of human Ribosomal Protein L15.
