Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit.
Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins.
RPL14 (ribosomal protein L14) encodes a ribosomal protein that is a component of the 60S subunit.
The protein belongs to the L14E family of ribosomal proteins.
It contains a basic region-leucine zipper (bZIP)-like domain.
The protein is located in the cytoplasm.
RPL14 contains a trinucleotide (GCT) repeat tract whose length is highly polymorphic; these triplet repeats result in a stretch of alanine residues in the encoded protein.
Transcript variants utilizing alternative polyA signals and alternative 5'-terminal exons exist but all encode the same protein.
As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of RPL14 dispersed through the genome.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity:Recognizes endogenous levels of human Ribosomal Protein L14. Species Crossreactivity: mouse and rat
Isotype:IgG
Calc Applications Abbrev:E WB
Calc Crossreactivity:Hu Mo Rt
Immunogen:Synthetic peptide corresponding to aa40-120 from the Internal region of human Ribosomal Protein L14.