RFC5 encodes the smallest subunit of the replication factor C complex, which consists of five distinct subunits (140kD, 40kD, 38kD, 37kD, and 36kD) and is required for DNA replication.
This subunit interacts with the C-terminal region of proliferating cell nuclear antigen and is required to open and load proliferating cell nuclear antigen onto DNA during S phase.
It is a member of the AAA+ (ATPases associated with various cellular activities) ATPase family and forms a core complex with the 38kD and 40kD subunits that possesses DNA-dependent ATPase activity.
A related pseudogene has been identified on chromosome 9.
Alternative splicing results in multiple transcript variants.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:5000-1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human RFC5. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to aa110-190 of human RFC5 protein.
