Transcription factors of the nuclear factor kappaB (NF-kappaB)/Rel family play a pivotal role in inflammatory and immune responses.
There are five family members in mammals: relA, c-Rel, RelB, NF-kappaB1 (p105/p50), and NF-kappaB2 (p100/p52).
Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively.
Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription.
In unstimulated cells, NF-kappaB is sequestered in the cytoplasm by IkappaB inhibitory proteins.
NF-kappaB-activating agents can induce the phosphorylation of IkappaB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-kappaB to enter the nucleus where it regulates gene expression.
NIK and IKKalpha (IKK1) regulate the phosphorylation and processing of NF-kappaB2 (p100) to produce p52, which translocates to the nucleus.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human RelB. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to human RelB around the non-phosphorylation site of S552.
