The Ras family small GTPase Ran is involved in nuclear envelope formation, assembly of the mitotic spindle and nuclear transport.
Like other small GTPases, Ran is active in its GTP-bound form and inactive in its GDP-bound form.
Nuclear RanGTP concentration is maintained through nuclear localization of guanine nucleotide exchange factor (GEF) activity, which catalyzes the exchange of bound GDP for GTP.
RCC1 (regulator of chromatin condensation 1) is the only known RanGEF.
RCC1 is dynamically chromatin-bound throughout the cell cycle, and this localization is required for mitosis to proceed normally.
Appropriate association of RCC1 with chromatin is regulated through N-terminal phosphorylation and methylation.
RCC1 regulation of RanGTP levels in response to histone modifications regulates nuclear import during apoptosis.
In mitosis RCC1 is phosphorylated at Ser11, possibly by cyclinB/cdc2.
This phosphorylation may play a role in RCC1 interaction with chromatin and its RanGEF activity.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:10,000ELISA: 1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous RCC1. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa301-350 from human RCC1.
