The protein encoded by RB1 is a negative regulator of the cell cycle and was the first tumor suppressor gene found.
The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure.
The active, hypophosphorylated form of the protein binds transcription factor E2F1.
Defects in RB1 are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.
RB1 (RB transcriptional corepressor 1) is a protein coding gene.
Diseases associated with RB1 include retinoblastoma and small cell cancer of the lung, somatic.
Among its related pathways are arrhythmogenic right ventricular cardiomyopathy (ARVC) and DNA Damage.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of Rb when Acetyl occurs at Lys873/874(human), Lys866/867(mouse) or Lys865/866(rat), while it doesn't react with total protein. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human Rb (Acetyl-K873/K874) protein at aaK873/872
