The protein encoded by RB1 (RB transcriptional corepressor 1) is a negative regulator of the cell cycle and was the first tumor suppressor gene found.
The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure.
The active, hypophosphorylated form of the protein binds transcription factor E2F1.
Defects in RB1 are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.
Applications:Suitable for use in Immunohistochemistry, Immunofluorescence, ELISA.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (paraffin): 1:100-1:300Immunofluorescence: 1:200-1:1000ELISA: 1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human Rb. Species Crossreactivity: Mouse, Rat
Isotype：IgG
Calc Applications Abbrev：E IF IHC
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human Rb around the non-phosphorylation site of S249.
