CCL5 (C-C motif chemokine ligand 5) is one of several chemokine genes clustered on the q-arm of chromosome 17.
Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes.
The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide.
This chemokine, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells and eosinophils.
It causes the release of histamine from basophils and activates eosinophils.
This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells.
It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor.
Alternative splicing results in multiple transcript variants that encode different isoforms.
Applications:Suitable for use in Immunohistochemistry, and ELISA.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (paraffin): 1:100-1:300ICC 1:200-1:1000ELISA: 1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human RANTES. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human RANTES at C-terminal.
