RAD50 double strand break repair protein encoded by RAD50 is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair.
RAD50 double strand break repair protein forms a complex with MRE11 and NBS1.
RAD50 double strand break repair protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends.
RAD50 double strand break repair protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination.
Knockout studies of the mouse homolog suggest RAD50 is essential for cell growth and viability.
Mutations in RAD50 are the cause of Nijmegen breakage syndrome-like disorder.
Applications:Suitable for use in Western Blot, Immunohistochemistry, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human Rad50. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa650-730 of human Rad50.
