RAD18, E3 ubiquitin protein ligase encoded by RAD18 is highly similar to S.
cerevisiae DNA damage repair protein Rad18.
Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA.
Similar to its yeast counterpart, RAD18, E3 ubiquitin protein ligase is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif.
Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human Rad18. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide corresponding to aa270-350 of human Rad18.
