84-5566-57　［取扱停止］Rad17 30ul　541511

特徴

• RAD17 checkpoint clamp loader component encoded by RAD17 is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage.
• RAD17 checkpoint clamp loader component shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs.
• RAD17 checkpoint clamp loader component binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage.
• RAD17 checkpoint clamp loader component recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation.
• The phosphorylation of RAD17 checkpoint clamp loader component is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells.
• Multiple alternatively spliced transcript variants of RAD17, which encode four distinct protein isoforms, have been reported.
• Two pseudogenes, located on chromosomes 7 and 13, have been identified.
• Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300Immunofluorescence: 1:200-1:1000ELISA: 1:10,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.