84-5564-40　［取扱停止］PTP1B, phosphorylated (Ser50) 100ul　541403

特徴

• The protein encoded by PTPN1 (protein tyrosine phosphatase, non-receptor type 1) is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence.
• PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues.
• Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity.
• PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation.
• This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase.
• This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation.
• Two transcript variants encoding different isoforms have been found for PTPN1.
• Applications:Suitable for use in Western Blot, Immunohistochemistry, and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.