84-5563-91　［取扱停止］PTEN, phosphorylated (Ser380/T382/T383) 100ul　541379

特徴

• PTEN (phosphatase and tensin homolog) was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency.
• The protein encoded by PTEN is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase.
• It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases.
• Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates.
• It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway.
• The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane.
• This longer isoform may help regulate energy metabolism in the mitochondria.
• A pseudogene of PTEN is found on chromosome 9.
• Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms.
• Applications:Suitable for use in Western Blot, Immunohistochemistry, and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:20,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.