PRC1 encodes a protein that is involved in cytokinesis.
The protein is present at high levels during the S and G2/M phases of mitosis but its levels drop dramatically when the cell exits mitosis and enters the G1 phase.
It is located in the nucleus during interphase, becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis.
This protein has been shown to be a substrate of several cyclin-dependent kinases (CDKs).
It is necessary for polarizing parallel microtubules and concentrating the factors responsible for contractile ring assembly.
Alternative splicing results in multiple transcript variants.
PRC1 (protein regulator Of cytokinesis 1) is a protein coding gene.
Among its related pathways are Semaphorin interactions and cytoskeletal signaling.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:10,000-1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human PRC1 phospho occurs at Thr481.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to human PRC1 (Phospho-Thr481) polyclonal. at aa481.
