The protein encoded by PLN (phospholamban)is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle.
The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein.
The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists.
The encoded protein is a key regulator of cardiac diastolic function.
Mutations in PLN are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure, and also familial hypertrophic cardiomyopathy.
Applications:Suitable for use in Immunofluorescence, ELISA.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:200-1:1000ELISA: 1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human PLB. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IF
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human PLB around the non-phosphorylation site of S16/T17.
