Eukaryotic translation initiation factor 2 alpha kinase 2 encoded by EIF2AK2 is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA.
The activated form of eukaryotic translation initiation factor 2 alpha kinase 2 can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis.
Eukaryotic translation initiation factor 2 alpha kinase 2 encoded by EIF2AK2 is also activated by manganese ions and heparin.
Three transcript variants encoding two different isoforms have been found for EIF2AK2.
Applications:Suitable for use in Western Blot, Immunohistochemistry, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human PKR.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to human PKR around the non-phosphorylation site of T446.
